Molecular Devices SpectraMax 340 PC 384 Microplate Reader. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeOpening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. The reaction mixture was incubated for 5 min. SOD activities were calculated from assays in which there was an inhibition of cytochrome c reduction of between 40 and 50%. , Sunnyvale, CA). Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays. #ABS PLUS) Methods. The microtiter plate was then incubated in the dark for 2 h at 37°C. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Percent Transmission Absorbance Competitor 0. SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer. Call 1-800-745-2710. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The intensity of red color formed in the assay was measured at a wavelength of 490 nm with an ELISA reader (SpectraMax 340, Molecular Device Co. , Oxon, UK). The concentration of NO in the cells was determined by measuring the amount of nitrite, a relatively stable oxidation product of. Molecular Devices SpectraMax M5e Microplate Reader. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). 5. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). D. Wallac 1450 Microbeta TRILUX Liquid Scintillation & Luminescence Counter $1,000. The drawer will open and it may close, if is doesn't, close it using the DRAWER button found on the Spectramax control panel (pictured in purple on. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. The SpectraMax 384 Plus lets you get absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. The chamber contains. The values were corrected for nonspecific background by subtracting the OD 650. CSF-1, IL-34 and calprotectin levels were analysed by ELISA according to instructions provided by the manufacturer (R&D Systems, Minneapolis, Minnesota, USA). The plate was read on a SpectraMax 340 PC plate reader (Molecular Devices, Sunnyvale, CA) at 450 nm, with reference at 650 nm. Absorbance at 490 nm was determined using a SPECTRAmax 340 (Molecular Devices Corporation). ThermoMax performs automatic mixing before and between readings to ensure reliable and precise. Cross sections of mouse skin was prepared and stained as previously described . Donor filter: 616/10 nm. D2. Features showcased by this model include. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. Photometric Range: 0. Molecular Devices. Quick View. #N207) LAL Reagent Water (Lonza cat. The protein concentration was determined spectrophotometrically at 280 nm in a 1 cm quartz cuvette using a SPECTRAmax-340 UV–vis spectrophotometer. The absorbance was read at the wavelength of 570 nm using a microplate spectrophotometer (SpectraMax 340, Molecular Devices, CA, USA). Like CD3, the CD95 death receptor requires binding of immobilized or multimeric antibodies to be activated effectively. Awareness Technology Inc. All absorbance measurements were taken on a SpectraMax 340 PC reader equipped with SoFTMax Pro software. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. ). 10× trypsin 0. FACS analysis. SpectraMAX 340. RESEARCH ARTICLE Effects of early-life conditions on innate immune function in adult zebra finches Merijn M. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. , Sunnyvale, CA, USA) at a wavelength of 450 nm. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. The concentration of Alizarin Red S staining in the samples was determined by comparing the absorbance values with those. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. Since the formation of NADPH is stoichiometrically related to the oxidation of glucose-6-phosphate, measurements for glucose + fructose can be determined by the absorbance of NADPH at 340 nm. Money Saved: $340. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. IMAP assays are based on binding of phosphate to immobilized metal coordination complexes on nanoparticles. Methods: We investigated growth inhibition by varying concentrations of AMB in seven isolates of C. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Manufacturer: Molecular Devices Model: SpectraMax The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. Absorbance was measured at 450 nm by using a SpectraMax 340 microplate spectrophotometer (Molecular Device). Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. 7 macrophages were obtained from ATCC (Manassas, VA). The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. StakMax. . Data are expressed as percent of maximum binding for each protein. After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San. One plate carrier adapter is provided with the instrument. 5. , Sunnyvale CA). Details. This was followed by an additional incubation at 37 °C for 30–60 min. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. 003 OD Stray light ≤ 0. 4. Softmax PRO v5. CD154 is an established indicator of antigen-reactive T helper cells in other species, but has not been characterized in horses. One plate carrier adapter is provided with the instrument. (1988) and recorded at 412 nm. With SOFTmax PRO, the contents of the wells in a microplate can be mixed SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). 5 ng/ml; estradiol: 10 pg/ml. The values were compared against a standard curve that was generated using known concentrations of IL6 to calculate concentration in the. 2 M DEEMM were mixed in a microtube,. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. SpectraMAX 340 plate reader (Molecular Devices). The adapter is. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. 3 mm. 1 mL of acetylacetone. Binding of capture antibodies to polystyrene beads and biotinylation. Wavelength(s) 340 to 850 nm; Add to Compare List. The optical density of each well was determined immediately using a microplate reader (SpectraMax-340, Molecular Devices) set to 450 nm. PathCheck SensorThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. 314 low lamp level warning. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. Quick View. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. 5. This reader only allows high performance HTRF measurement when assays are run in. 1. 00. SpectraMax Multi-Mode Microplate Readers. 0 OD. The metals were preincubated with A for 5 min when added before heme. This test was performed in duplicates. The cell growth was determined by measuring the optical density at 600 nm using a spectrophotometer (SpectraMax 340, Molecular Devices, USA). This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. 306 plate air cal. 3. Cell permeabilization. The VersaMax and SpectraMax 190 read 96-well microplates. The inhibition of ALDH activity by compounds and the IC50 curves were determined by measuring the formation of NAD(P)H spectrophotometrically at 340 nm (molar extinction coefficient of 6200 M −1 cm −1) on a Beckman DU-640 as well as a Spectramax 340 PC spectrophotometer (GMI, Ramsey, NJ, USA) using a purified. SpectraMax 340 (Obsolete), SpectraMax 340PC 384 (Obsolete), Gemini EM reader, SpectraMax 250 (Obsolete) What is h the bandwidth for SpectraMax® plate readers and how do we determine it? The instrument bandwidth for the reader is expressed as half-bandwidth, meaning the full. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. Plasma glucose levels were measured using FreeStyle Lite (Abbott Diabetes Care Ltd. 5 stars - 1602 reviewsThis assay typically develops in 1. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. Assay setup for a 384-well low-volume plate. Microplate Readers. Percent viability was calculated by comparison with the control group. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. 1 mM MnCl2 and 25 mM CHES buffer, pH 9. Labsystems Luminoskan RT Microplate Reader. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. 340 nm / 80 nm. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. 5 mL of 1 M acetate buffer (pH 4. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. The SpectraTest validation package provides a NIST-traceable solution for validating optical performance of the SpectraMax Plus 384 reader and does it automatically. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. 1 0 100 80 60 40 20 0 4. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. After treatment, total cellular RNA was extracted with a cold RNA extraction solution (Ultraspec RNA;Call 1-800-745-2710. The GH Pro 340 Supplemental Greenhouse LED grow lighting is designed with greenhouse operations in mind. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. One plate carrier adapter is provided with the instrument. See More Microplate Reader / Microtiter Plate Reader. 1 All Necessary Cables and Cords Manuals General Specifications Dimensions (in. Absorbance values were measured using a plate reader (Molecular Devices; SpectraMax 340) at λ = 405 nm. 5. 1 mL of culture media with 10% FBS. Amino Acid Analysis. Lactate Dehydrogenase (LDH) Release. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects andSPECTRAmax ® PLUS 384 Microplate Spectrophotometer. The results are expressed as the percentage inhibition of BrdU incorporation compared to the untreated control. Customer Transformation Hub. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. Although all samples initially were analyzed at the same volume, the volume of sample. Immunofluorescence and Aggregation Analysis. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. The first time you replace an OEM lamp you must use the N-34-MLA. Microplate Spectrophotometers. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. Cell Assay Cells (5 × 10 5 per well) are plated in triplicate in 96-well plates in 0. SpectraMax Injector Cartridge with SmartInject™ Technology. 0 1. Hydrogen peroxide (H 2 O 2) was used to establish an intracellular oxidative. 340/70 nm. Includes: SpectraMax 340PC384. SpectraMAX 340. VersaMax reads 96-well and strip-well plates. The ratio of red to green fluorescence intensity was calculated. Total Cell Count in BALF Cells collected from the lavage fluid were suspended in 1 mL of F-12 media supplemented with 10% FBS. 2. Subsequent replacements require only the lamp (N-34-LB) be replaced. Page: 1/6. application or budget. The contents of the. Crowder, Ziqian Zeng, Alissa N. 5. used. 340 nm / 70 nm. 5 mm (bottom read) Beam diverges to focal spot near bottom of well. Cell viability wasDescription Microplates The SpectraMax Multi-Mode Microplate Reader can accommodate SBS- standard 6-well to 384-well microplates and strip wells. The narrow bandwidth used by the model increases measurement accuracy and broad analysis over. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. reader (Molecular Devi ces). In vivo anti-malarial assay in mice. The extreme flexibility and high sensitivity of the SpectraMax GEMINI EM make it appropriate for applications within the fields of bio-A microplate reader that can detect two or more applications is considered a multi-mode plate reader. 1 mL of culture media with 10% FBS. 85%. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Please refer to photos for further. Analysis of ALA by Ehrlich’s Reagent Assay. 2 g/L solution; dilute 1:10 in HBSS. Description 1. With SOFTmax PRO, the contents of the wells in a microplate can be mixed used in the SPECTRAmax PLUS. 04. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. more on but is otherwise untested and sold AS-IS. We also offer depot Repair Service from our base of operations in Southern California (San Diego). Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. 1 mg mL −1 CDDP loaded A-CHAM, B-CHAM, C-CHAM or D-CHAM. 2. 30. Laser Scanning Confocal Microscopy The fluorescent signals were obtained using a Zeiss LSM 510 laser scanning confocal microscope. This is a used Molecular Devices SpectraMax 340PC Microplate Spectrophotometer. TheSpectraMax®Plus384addstheabilitytoread SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. 4. The mixture changed to a red color by a reaction of LDH that was measured by a plate reader (SpectraMax 340 PC; Molecular Devices, San Jose, CA, USA) at a visible wavelength of. 4) was followed according to Vanacker et al. TGFα release was calculated as described in the Results. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance. Alves, Amelia K. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. 1 in stock! Molecular Devices SpectraMax M2 Multilabel Microplate Reader. The OD 490 was measured with an absorbance microplate reader (SpectraMax 340; Molecular Devices). Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. A 340 μL of 50 mM borate buffer (pH 9), 240 μL methanol, 10 μL standard solution or sample, and 20 μL of 0. FTIR Spectrometer: Shimadzu IRSpirit (2018)The data were acquired with a Molecular Devices SpectraMax 340 Microplate Reader (Sunnyvale, CA, USA). 30 µs. SpectraMax Multi-Mode Microplate Readers. PerkinElmer offers a variety of Microplates For Absorbance and Colorimetric Assays. The amount of liberated pNA product during incubations was determined using a standard curve obtained at 410 nm for various concentrations of a pNA stock solution in the SpectraMax 340 microplate reader. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. 3 0. Permeability of the monolayer was verified by adding 1% Evans Blue dye (Thermo Fisher Scientific, Inc. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). Absorbance was read at 450 nm using a Molecular Device Spectramax 340. SPECTRAmax® 190. 313 reference gain check fail. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. Stat Fax 4700 Microstrip Reader. SpectraMax 250 With Computer SoftwareSpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Gerritsma,B. After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. ATI/240, 250. Six wells were assayed for each set of conditions and stadard deviations. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. 303 unable to cal dark current. The antibodies originally defining this molecule by inducing apoptosis, anti-Fas and anti-APO-1 , were of the IgM or IgG3 subtype, respectively. Filipin StainingSpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. measurements were performed on a SPECTRAmax-340 plate reader (Molecular Devices Inc. StakMax Microplate Handling Stacker. Immunofluorescence and Aggregation Analysis. Each experiment was performed in duplicate and repeated at. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 0% and ± 0. These isotypes cause antigen cross-linking by multimeric binding and self. Capable of detecting DNA down to 25 ng per well, this model gives users the same sensitivity as conventional systems to produce accurate nucleic acid measurements. The cytotoxicity of the CDDP loaded CHAMs were done by treating the FaDu cells with DMEM supplemented with 0. The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. , Sunnyvale, CA, USA) at 595 nm. The SPECTRAmax 340PC can mea-The extracted stain was then transferred to a 96-well plate, and the absorbance at 562 nm was measured using a SpectraMax 340 plate reader/spectrophotometer(Molecular Devices Corp. Absorbance was recorded at 540 nm using SpectraMAX 340 reader. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. The VersaMax and SpectraMax 190 read 96-well microplates. Following incubation for 30 min at room temperature, the reaction was quenched by addition of 100 μl stop solution (2 N H 2 SO 4) and absorbance was read using microplate reader (SpectraMax 340; Molecular Devices Co. Glutathione reductase (GR) activity was measured, according to Smith et al. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. MCE has not independently confirmed the accuracy of these methods. Following cell culture under normoxic or hypoxic con-ditions, culture medium samples were obtained on completing the incubation period to measure atrial natriuretic peptide. The optical system of the SpectraMax 340PC Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). The plate was covered and incubated for one hour at room temperature. reader (SpectraMax 340; Molecular devices, LLc). The sensitivities of the hormones are Prolactin: 0. The plates were incubated for 4 additional hours at 37°C. density was measured at 570 nm (SpectraMax 340, Molecular De-vices, Sunnyvale, CA). Not all manufacturers' microplates are the same with regard to design, materials, or76358-624. Analysis of ALA by Ehrlich’s Reagent Assay. The SpectraMax® iD3 Multi Mode Microplate . The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Data System. The assays contained 0. , Sunnyvale, CA, USA) at 595 nm. Immunofluorescence and Aggregation Analysis. Cell proliferation was measured using the MTT method. Plate reader (SpectraMax 340 PC plate reader or comparable plate reader). 2. plates can be used in the SPECTRAmax PLUS 384. Acceptor filter: 665/10 nm. ). , Sunnyvale, CA). The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The SpectraMax™ GEMINI EM Dual-Scanning Microplate Spectrofluorome-ter can perform a variety of fluorescent applications as well as some lumines-cent assays. Statistical analysis The significant differences between the different strains in the adherence assays were analyzed using the Student’s t -test. Emission. 5 mL sample or standard solution was well mixed with 0. Table 1. 315 can't find zero order. 5, 2 mM pNPP, and recombinant enzymes, PTP1B activities were continuously monitored on a SpectraMax 340 Microplate Reader (SpectraMax, Silicon Valley, CA, USA) at 405 nm for 2 min at 30°C. Spectral Range. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices) Methods VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. MMP‑1 inhibition assay. 304 signal level saturation. Call 1-800-745-2710. No power cord or accessories included. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Page 1 ® SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0049 Rev. edu Contact Phone: 216-368-8962. Values were determined according to a standard curve (log/log curve-fit). Sandwich ELISAs were used to measure insulin (DY8056, R&D Systems, Minneapolis, MN, USA). Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Intra- and inter-assay coefficients of variation were lower than 8. Analytical Data Regression and Acceptance Limits The conversion of the optical density units for the test samples, VSs, and QCs to concentrations was performed using the Watson LIMS software (version. 6) and 0. a SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). Centrifuge – Eppendorf 5417C with rotor FA 45–30–11. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. About The SpectraMax GEMINI EM is controlled by an external computer running SoftMax ® Pro software which provides integrated instrument control, data display, and statistical data analysis. BV-2 cells were treated with various concentrations of BT and/or LPS for 24 hFollowing incubation, absorbance was immediately measured at 405 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, LLC. well, and strip-well microplates. 313 reference gain check fail. Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. Call 1-800-745-2710. VersaMax reads 96-well and strip-well plates. ATI Note: #1: The N-34-MLA is a replacement assembly which replaces an OEM lamp. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. #PLUS 384) MethodsSpectraMax 340 PC, Molecular Devices, USA was used as a 96-well microplate reader to test bioactivities. SpectraMax Multi-Mode Microplate Readers. , Fremont, CA, USA). This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Microplate Readers. The SpectraMax® Gemini™ EM Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. Products : SpectraMax Paradigm reader, SpectraMax Plus 384 reader, SpectraMax 250 (Obsolete), SpectraMax L reader, SpectraMax 340PC 384 (Obsolete), Gemini EM reader, Gemini XPS reader, SpectraMax 340 (Obsolete). SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Plasminogen was added to the fibrinogen before addition of thrombin and either tissue plasminogen activator (tPA) or urokinase plasminogen activator in the. Reagena Poc Puumala® was used for detection of IgM antibody against PUUV in NE patient sera at the Department of Clinical Virology,. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. . 4. The control panel consists of a 2-x-20-character LCD and eleven pressure-sensitive membrane keys that can be used to control some functions of the instrument. Example: [Drawer]. In this study we used tetanus toxoid (TT) as a well-defined antigen to. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Background: A computerized machine, SPECTRAmax 340, was used to evaluate the recently reported phenomenon of antagonism of the polyene amphotericin B (AMB) in Candida albicans pre-exposed to the triazole fluconazole (FLZ). Anti-proteolytic activity. Male 8 pin mini Din to Female 9D. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. 0200-7027. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. Absorbance values were read at 570 nm on an automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA). Cell viability was determined using the blue formazan assay in which colorless 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) is metabolized to a blue product by mitochondrial dehydrogenases. spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software, metamorph software, spectramax i3 platform. Molecular Devices SpectraMax Gemini XS Microplate Reader. Novak, Nathan J. 5 g/L-EDTA 0. 5), L-ascorbic acid (25. Not all manufacturers' microplates are the same with regard to design, materials, orAbsorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. The absorbance at 450 nm was determined using a microplate reader (spectraMAX 340). 0 0 The indicated microplates were scanned form 200 nm to 800 nm. Stat Fax 4700 Microstrip Reader. MOLECULAR DEVICE M5. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. Incubate 30 min at room temperature, and then read microplates at 405 nm using SpectraMax 340 PC plate reader (Molecular Devices) (see Note 8). Dissolve 13.